Role of the Wnt/β-Catenin Signaling Pathway in Reducing Lipogenesis of Obese Rats Under Hypoxic Exercise

Hypoxic exercise exerts a significant effect on body weight loss and fat reduction. Methods: In the present study, we constructed an obese rat model divided rats into following groups: Normoxic quiet (C), (H), Exercise (CE), (HE). We employed isobaric tags for relative absolute quantification (iTRAQ) using proteomic technology to screen differentially expressed proteins in four groups. Additionally, used Reduced-Representation Bisulfite Sequencing (RRBS) analyze high-throughput DNA methylation profiles both HE CE Finally, screened analyzed CpG islands Differentially Methylated Regions (DMRs) of genes related Wnt/β-catenin-signaling pathway. Results: The mRNA levels gene products pathway (i.e., c-myc β-catenin) were significantly augmented (P<0.05) group compared group; APC GSK3β diminished (P<0.05). expression β-catenin also increased Among Wnt-signaling pathway, CpG-promoter sites included Fzd2, Fzd9, Wnt16, Wnt2b, Sfrp2, Sfrp1, Sox2, Sox1; DMRs CDC, Fzd10, Gsk-3β, PI3k, Wnt16 Wnt6. Conclusions: Our findings provide evidence that hypoxic may inhibit adipogenesis through canonical Wnt signaling.